HEK293 cell lysate with overexpressed SPL-GFP (green fluorescent protein).

Storage: <-80°C. The enzyme is stable for 6 months from the date of receipt. Avoid repeat freeze thaw cycles.

Product Description

Sphingsoine 1-phosphate lyase (SPL) (E.C.4.1.2.27) works as a key regulator of the final step of the sphingolipid degradative pathway by irreversibly breaking down the sphingosine-1-phosphate (S1P) to ethanolamine phosphate and hexadecenal. S1P signaling through its five receptors involves in many biological events such as proliferation, inflammation and apoptosis in cancer and other degenerative diseases.

A support protocol for detection of S1P lyase activity is available below.

References

1. Billich, A., et al. (2013). “Partial deficiency of sphingosine-1-phosphate lyase confers protection in experimental autoimmune encephalomyelitis.” PLoS One 8(3): e59630.2. Engel, N., et al. (2012). “Metabolic profiling reveals sphingosine-1-phosphate kinase 2 and lyase as key targets of (phyto-) estrogen action in the breast cancer cell line MCF-7 and not in MCF-12A.” PLoS One 7(10): e47833.3. Bedia, C., et al. (2009). “Synthesis of a fluorogenic analogue of sphingosine-1-phosphate and its use to determine sphingosine-1-phosphate lyase activity.” Chembiochem 10(5): 820-2.4. Reiss,U., et al. (2004) “Sphingosine-phosphate Lyase Enhances Stress-induced Ceramide Generation and Apoptosis.” J. Biol. Chem. 279(2): 1281-1290.

Echelon-脂类研究的最佳拍档

Echelon是脂质研究和脂质与蛋白质相互作用研究领域的引导者。脂质蛋白相互作用是众多的细胞信号通路钟高级调控必不可少的。异常的细胞信号通路将会导致许多疾病，例如癌症、炎症、糖尿病和心血管疾病等。Echelon一直是脂质研究信息，脂质信号分析的顶级供应商。