Z-R015 is an affinity purified rabbit polyclonal anti-hVps34 antibody.  It can immunoprecipicate human Vps34 from cell lysate without inhibition.  There is no need to elute the enzyme prior to running and activity assay.  See figure above for hVps34 activity data obtained using Z-R015 in IP. 

Antigen: human Vps34 (PIK3C3, Class III PI3-Kinase). 

For Western Blot application and immunostaining, we recommend anti-hVps34 antibody, cat# Z-R016. 

Concentration

250 µg/mL

Formulation

Antibody is formulated in 100 mM Tris-HCl, 100 mM glycine, pH 7.5, and 50% glycerol.

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Publications

1. Singla A, Fedoseienko A, Giridharan SSP, Overlee BL, Lopez A, Jia D, et al. (2019) Endosomal PI(3)P regulation by the COMMD/CCDC22/CCDC93 (CCC) complex controls membrane protein recycling. Nature Communications.2. Scotto Rosato A, Montefusco S, Soldati C, Di Paola S, Capuozzo A, Monfregola J, et al. (2019) TRPML1 links lysosomal calcium to autophagosome biogenesis through the activation of the CaMKKβ/VPS34 pathway. Nature Communications.3. Su H, Yang F, Wang Q, Shen Q, Huang J, Peng C, et al. (2017) VPS34 Acetylation Controls Its Lipid Kinase Activity and the Initiation of Canonical and Non-canonical Autophagy. Mol Cell.

Echelon-脂类研究的最佳拍档

Echelon是脂质研究和脂质与蛋白质相互作用研究领域的引导者。脂质蛋白相互作用是众多的细胞信号通路钟高级调控必不可少的。异常的细胞信号通路将会导致许多疾病，例如癌症、炎症、糖尿病和心血管疾病等。Echelon一直是脂质研究信息，脂质信号分析的顶级供应商。