CatalogNo.: K-4000E Description: Echelon'sPI(4)PMassELISAkitisdesignedtodetectandquantifyPI(4)PbymeansofacompetitiveELISAformat,eliminatingtheneedforrADIoactivityandthinlayerchromatography.ThePI(4)PMassELISAdirectlydetectsPI(4)PoverallotherphosphonositidesatrelevantBIOLOGicallevels. AssayRange:50-0.7pmolPI(4)P SampleType:Drieddownlipidextractedfromcells SampleVolume:60μL/sample,singlepoint | |||
ProductBackground:ThePI(4)PMassassayisacompetitiveELISAinwhichthesignalisinverselyproportionaltotheamountofPI(4)Pmeasured.ThePI(4)PextractionsareaddedtothecoloredmixingplatewithPI(4)PGrip.ThismixtureisthentransferredtothePI(4)PDetectionPlateforcompetitivebinding.Aperoxidase-linkedsecondarydetectorandcolorimetricdetectionisusedtodetecttheamountofPI(4)PGripbindingtotheplate.Theassayissensitiveto0.7pmolPI(4)P. StorageThekitcomesintwopartswithdifferentstoragerequirements.UponreceiptstoreKitPart1at4°CandKitPart2at-20°C.Storepreparedreagentsasindicatedintheprotocol.FeaturedinReferences1)S.Choi,etal."AgoNIST-stimulatedphosphatidylinositol-3,4,5-trisphosphategenerationbyscaffoldedphosphoinositidekinases"NatureCellBiology(2016)18(12),1324-35,doi:10.1038/ncb3441RelatedProductsEchelon'sPIPMassAssaysPIP3MassELISA PI(4,5)P2MassELISA PI(3,4)P2MassELISA PI(3)PMassELISA PIK-93(PI4KIIIbinhibitor) |
Echelon-脂类研究的最佳拍档
Echelon是脂质研究和脂质与蛋白质相互作用研究领域的引导者。脂质蛋白相互作用是众多的细胞信号通路钟高级调控必不可少的。异常的细胞信号通路将会导致许多疾病,例如癌症、炎症、糖尿病和心血管疾病等。Echelon一直是脂质研究信息,脂质信号分析的顶级供应商。