Sphingosine-fluorescein is an alkyl-labeled sphingosine synthesized with the natural erythro stereochemistry that maintains its natural properties.

Echelon offers a unique selection of sphingosine analogs labeled at the terminus of the alkyl chain with biotin or fluorescent groups. All compounds are synthesized from chiral precursors and are available as lyophilized powders. Echelon’s alkyl-labeled sphingosines are synthesized with the natural erythro stereochemistry and maintain similar properties as sphingosine. For example, labeled sphingosines are phosphorylated by sphingosine kinases (1 & 2) and can be used in high throughput screening applications. In addition, they induce a Nieman Pick type C1 disease cellular phenoype in RAW macrophages in the same manner as exogenously added sphingosine.

Excitation/Emission 494/521 nm

Bulk quantities of this product are available. Please email echelon@echelon-inc.com for more information.

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Featured in Publications

1) Lloyd-Evans, E., A. J. Morgan, et al. (2008). “Niemann-Pick disease type C1 is a sphingosine storage disease that causes deregulation of lysosomal calcium.” Nat Med 14(11): 1247.2) Xiang, Y., G. Asmussen, et al. (2009). “Discovery of novel sphingosine kinase 1 inhibitors.” Bioorg Med Chem Lett 19(21): 6119-21.3) Caliper Life Sciences Application Note 2054) Peest, U., S. C. Sensken, et al. (2008). “S1P-lyase independent clearance of extracellular sphingosine 1-phosphate after dephosphorylation and cellular uptake.” J Cell Biochem 104(3): 756-72.5) Meissner, A., J. Yang, et al. (2012). “Tumor Necrosis Factor-α-Mediated Downregulation of the Cystic Fibrosis Transmembrane Conductance Regulator Drives Pathological Sphingosine-1-Phosphate Signaling in a Mouse Model of Heart Failure.” Circulation 125(22): 2739-2750.

Echelon-脂类研究的最佳拍档

Echelon是脂质研究和脂质与蛋白质相互作用研究领域的引导者。脂质蛋白相互作用是众多的细胞信号通路钟高级调控必不可少的。异常的细胞信号通路将会导致许多疾病，例如癌症、炎症、糖尿病和心血管疾病等。Echelon一直是脂质研究信息，脂质信号分析的顶级供应商。