Recombinant anti-Focal Adhesion Kinase Nanobody, Clone # Nb54, COOH-terminal HA tag.Type: VHH single domainSource: E. ColiApplications: ELISA, Immunoprecipitation, Pull downClonality: Monoclonal

Background: Focal Adhesion Kinase VHH Antibody was produced in a llama with a protein fragment encompassing mouse FAK amino acids 750-1053 then cloned for recombinant production in E. Coli. FAK is a cytosolic tyrosine kinase of 125 kDa that concentrates in focal adhesions. It contains FERM (4.1 protein, Ezrin, Radixin, Moesin) and kinase domains that form an autoinhibitory association and a FAT (focal adhesion targeting domain) domain that localizes it to focal adhesions. FAK promotes cell migration by activating a signaling pathway that induces turnover of cell contacts with the underlying matrix.

Recombinant anti-Focal Adhesion Kinase Nanobody 54 was developed by Gulliver Biomed.

Storage

Store at -20°C upon arrival. For long term storage, aliquot and store at -80°C. Avoid repeated freeze/thaw cycles.

Publications

1) Van Audenhove I, Debeuf N, Boucherie C, Gettemans J. (2015) Fascin actin bundling controls podosome turnover and disassembly while cortactin is involved in podosome assembly by its SH3 domain in THP-1 macrophages and dendritic cells. Biochim Biophys Acta; 1853(5):940-952.

Keywords: focal adhesion kinase, FAK, nanobody, VHH, antibody, single domain

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Echelon-脂类研究的最佳拍档

Echelon是脂质研究和脂质与蛋白质相互作用研究领域的引导者。脂质蛋白相互作用是众多的细胞信号通路钟高级调控必不可少的。异常的细胞信号通路将会导致许多疾病，例如癌症、炎症、糖尿病和心血管疾病等。Echelon一直是脂质研究信息，脂质信号分析的顶级供应商。