Recombinant anti-Talin Nanobody, Clone # Nb19, COOH-terminal HA tag.Type: VHH single domainSource: E. ColiApplications: ELISA, Immunoprecipitation, Pull downClonality: Monoclonal

Background: Talin VHH Antibody was produced in an llama with a fragment of mouse Talin encompassing residues 2364-2541 then cloned for recombinant production in E. Coli. Talin is a ~270 kDa mechanosensitive cytoskeletal protein that links the actin cytoskeleton to integrins. It is concentrated in regions where cells make contact with the substrate or with other cells such as focal adhesions, immune synapses and invadopodia or podosomes.

Recombinant anti-Talin Nanobody 19 was developed by Gulliver Biomed.

Storage

Store at -20°C upon arrival. For long term storage, aliquot and store at -80°C. Avoid repeated freeze/thaw cycles.

Publications

1) Van Audenhove I. et al. (2014) Stratifying facsin and cortactin function in invadopodia formation using inhibitory nanobodies and targeted subcellular fractionation. FASEB J; 28(4):1805-18182) Van Audenhove I. et al. (2015) Facsin actin bundling controls podosome turnover and disassembly while cortactin is involved in podosome assembly by its SH3 domain in THP-1 macrophages and dendritic cells. Biochem Biophys Acta; 1853(5):940-952

Keywords: Talin, Src kinase, nanobody, VHH, antibody, single domain

‘Nanobody’ and ‘nanobodies’ are registered trademarks of Ablynx N.V.

Echelon-脂类研究的最佳拍档

Echelon是脂质研究和脂质与蛋白质相互作用研究领域的引导者。脂质蛋白相互作用是众多的细胞信号通路钟高级调控必不可少的。异常的细胞信号通路将会导致许多疾病，例如癌症、炎症、糖尿病和心血管疾病等。Echelon一直是脂质研究信息，脂质信号分析的顶级供应商。