Sphingo Arrays are membranes pre-spotted with a concentration gradient of all eight sphingolipids.  Sphingo Arrays are used in a simple protein-lipid overlay assay to help determine the specificity and sensitivity of your protein towards one or more of these lipids.

Briefly, once the membrane is blocked, it is incubated with your protein of interest.  The membrane is then washed and incubated with an HRP labeled antibody against the protein or your proteins affinity tag. The membrane is washed again before incubation with ECL or a precipitating TMB detection reagent. This can then be visualized with the appropriate equipment based on the detection reagent used.

Sphingo Arrays are a 4 x5 cm hydrophobic membrane with 100, 50, 25, 12.5, 6.25, 3.13, and 1.56 pmol of the following sphingolipids: Sphingosine, Sphingosine 1-phosphate (S1P), Sphingosylphosphorylcholine (SPC), Phytosphingosine, Ceramide, Sphingomyelin, Sulfatide, Monosialoganglioside-GM1.

Echelon-脂类研究的最佳拍档

Echelon是脂质研究和脂质与蛋白质相互作用研究领域的引导者。脂质蛋白相互作用是众多的细胞信号通路钟高级调控必不可少的。异常的细胞信号通路将会导致许多疾病，例如癌症、炎症、糖尿病和心血管疾病等。Echelon一直是脂质研究信息，脂质信号分析的顶级供应商。