VitaBlueisaproprietaryfluorometric/colorimetricgrowthindicatorbasedondetectionofmetabolicactivityofcellls. Itisnon-toxictocellsandmeasurestheoxidation-reduction(redox)environmentinthemedium.ViablecellsreduceVitaBluefromitsoxidizedform(nonfluorescent,bluecolor)tothereducedform(fluorescent,redcolor)duringcellgrowth. ThefluorescenceintensityofVitaBlueat540ex/590emis proportionaltothenumberofviablecellsinthesample.Ifafluorescenceplatereaderis unavailable,absorbancedifferenceat570nmand600nmcanbeusedinstead. Pleaseseegraphsbelowforexample. VitaBlueissuitableforvarioushuman/animalandplantcelllines,bacteria,mycobacteria,andfungi. ItcanbeusedtoassesscellviABIlity,cellproliferation,andcytotoxicity.Itisnontoxic,simpletouse,sensitive,stable,andhighlyreproducIBLe.

Storage

• Lightsensitive,storetightlycappedinthedarkat4-22°Cfor12months.VitaBluecanbestoredat-20°Ctoextendtheshelflife.Ifstoredfrozen,ensureallcomponentsareinsolutionbywarmingto37°Candmixingasnecessarybeforeuse.

SuggestedUse

• VitaBlueisagreattoolincancerresearch,cellviabilitystudy,proliferationstudy,drugcytotoxicitystudy,andcellgrowthrelateddrug/inhibitor/stimulatorscreens.