echelon-inc/PTEN Activity ELISA/1kit/K-4700-1kit,Echelon,,Echelon,Echelon Biosciences Incorporated,echelon-inc,EBI,生命科学Echelon Biosciences Incorporated价格优惠 echelon-inc一级代理,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：echelon-inc/PTEN Activity ELISA/1kit/K-4700-1kit

产品编号：K-4700-1kit

市场价：¥11900.00

场地：美国(厂家直采)

产品分类：试剂盒>ELISA试剂盒>夹心法ELISA>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$595.00

品牌： Echelon

公司：Echelon Biosciences Incorporated (EBI)

公司分类：

商品介绍

CatalogNo.: K-4700

Description: ThePTENActivityELISAisdesignedtoquantifythephosphataseactivityofPTENbydetectionoftheproduct,PIP₂,inacompetitiveELISAformat,eliminatingtheneedforrADIoactivity,organicsolvents,andthinlayerchromatography.TheassaycanbeusedtomeasurerecombinantorimmunoprecipitatedPTENactivity.

PTEN(PhosphataseandTensinHomologdeletedonChromosome10)isa3’phosphoinositidephosphatasethatconvertsPI(3,4,5)P₃toPI(4,5)P₂,thusopposingPKB/AktactivationbyPI3-K.PTENisinvolvedinneuronalstemcellproliferationandself-renewal,cardiacmyocytehypertrophyandcontractility,andawiderangeofdevelopmentalprocesses.PTEN,however,isbestknownforitsroleasatumorsuppressor.LossofPTENactivityresultsinaccumulationofPI(3,4,5)P₃,abnormalactivationofPKB/Akt,unregulatedcellgrowth,suppressionofapoptosis,andincreasedtumOrigenesisinanumberofhumantissues.IthasalsobeenproposedthatPTENisacandidatefortargetedchemotherapybecausecertainanti-canceragentspreferentiallydestroytumorswithPTENmutations.Inadditiontothisdirectroleincancer,PTENhasrecentlybeenshowntoregulatecancer-associatedpathwaysincludingVEGF-mediatedangiogenesisandothers.
ThePTENActivityELISAdirectlydetectsthephosphoinositideproductcomparedtootherassays,whichdetectfreephosphate.ThiseliminatesmanypossIBLesourcesoferrorduetothefactthatinorganicphosphateistheproductofmanyphosphataseenzymeactivities,andisfoundincommonbuffersandcleaningproducts.TheassayisacompetitiveELISAinwhichthesignalisinverselyproportionaltotheamountofPI(4,5)P₂produced.AfterthePTENreactionsarecomplete,reactionproductsareaddedtothePI(4,5)P₂-coatedmicroplateandaPI(4,5)P₂detectorproteinisthenaddedforcompetitivebinding.Aperoxidase-linkedsecondarydetectorandcolorimetricdetectionisusedtodetectPI(4,5)P2detectorbindingtotheplate.ThecolorimetricsignalisinverselyproportionaltotheamountofPI(4,5)P2producedbyPTEN.

Storage

Part14°C,Part2-20°C

FeaturedinPublications

1)Tzenaki,N.,M.Aivaliotis,etal.(2015)."Focaladhesionkinasephosphorylatesthephosphataseandtensinhomologdeletedonchromosome10underthecontrolofp110Î´phosphoinositide-3kinase."FasebJ.doi:10.1096/fj.15-274589
2)Guo,S.T.,M.N.Chi,etal.(2015)."INPP4Bisanoncogenicregulatorinhumancoloncancer."Oncogene.doi:10.1038/onc.2015.361

RelatedProducts:

PTENenzyme(E-3000)
MalachiteGreenAssayKit(K-1500)
PTENSubstrates:P-3908,P-3916,Q-1345
SF1670(PTENInhibitor)(B-0350)
OtherLipidPhosphatases:SHIP2(E-1000),INPP4A(E-8000)

品牌介绍

Echelon-脂类研究的最佳拍档

Echelon是脂质研究和脂质与蛋白质相互作用研究领域的引导者。脂质蛋白相互作用是众多的细胞信号通路钟高级调控必不可少的。异常的细胞信号通路将会导致许多疾病，例如癌症、炎症、糖尿病和心血管疾病等。Echelon一直是脂质研究信息，脂质信号分析的顶级供应商。

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