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产品名称：echelon-inc/INPP4 Activity Kit/1kit/K-5200-1kit

产品编号：K-5200-1kit

市场价：¥9980.00

场地：美国(厂家直采)

产品分类：生化试剂>脂质代谢研究>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$499.00

品牌： Echelon

公司：Echelon Biosciences Incorporated (EBI)

公司分类：

商品介绍

CatalogNo.: K-5200

Description: Echelon’sINPP4ActivityAssayisacompetitiveELISAwhichmeasurestheactivityofINPP4AandINPP4B.

AssaySensitivity:Theassaycanmeasureaslittleas25pmolPI(3)Pina100µLdetectionmixture.

INPP4Aisamagnesium-independentphosphataseinvolvedinthephosphatidylinositol3-kinase(PI3K)pathway.INPP4AanditsisoenzymeINPP4Bhydrolyzethe4-phosphatefromthelipidsecondmessengerphosphatidylinositol-3,4-bisphosphate(PI(3,4)P2)toformphosphatidylinositol-3-phosphate(PI(3)P).Whilelittleevidencesupportsitsroleasatumorsuppressor,INPP4AsuppressesglutamateexcitotoxicityinthecentralnervoussystemandisresponsIBLefortheWeeblemousephenotype.AspontaneousframeshiftmutationintheInpp4ageneispresentintheWeeblemouseresultinginselectiveneuronallossinthecerebellumresulting,severeataxiaandearlydeath.
TheINPP4ELISAisacompetitiveassayinwhichthesignalisinverselyproportionaltotheamountofPI(3)Pproduced.AftertheINPP4reactionsarecomplete,reactionproductsareaddedtothePI(3)P-coatedmicroplateforcompetitivebindingtoaPI(3)Pdetectorprotein.TheamountofPI(3)Pdetectorproteinboundtotheplateisdeterminedthroughcolorimetricdetection.Theassaydisplaysexcellentrobustness(Z’value~0.8).