 | CatalogNo.: K-1800 Description: Echelon’sNeutralSphingomyelinase(N-SMase)AssaykitisanenzymecoupledassaythatmeasuressphingomyelinaseactivityinBIOLOGicalsamplesthroughthedownstreamproductionofcholine. AssayIncubationtime:2-4hours AssayRange:5mU/mL-0.078mU/mL SampleType:tissuehomogenates,celllysates,serum,saliva,andurine | ||
ProductBackground:Inthisenzymecoupledassay,NeutralSphingomyelinase(N-SMase)catalyzesthehydrolysisofsphingomyelinintophosphorylcholineandceramide.Alkalinephosphatasethencatalyzesphosphorylcholinetocholine.CholineisthemoxidizedbyCholineoxidaetoformhydrogenperoxide.Finally,DAOSplus4-Aminoantipyrine(4AAP)inthepresenceofhydrogenperoxideandperoxidaseresultsintheoxidativecouplingofDAOSand4AAPtoformabluechromogenthatisdetectedbymeasuringtheabsorbanceoflightat595nm. Echelon-脂类研究的最佳拍档 Echelon是脂质研究和脂质与蛋白质相互作用研究领域的引导者。脂质蛋白相互作用是众多的细胞信号通路钟高级调控必不可少的。异常的细胞信号通路将会导致许多疾病，例如癌症、炎症、糖尿病和心血管疾病等。Echelon一直是脂质研究信息，脂质信号分析的顶级供应商。 | |||
