ProductBackground:Sphingomyelinasescatalyzesthehydrolysisofsphingomyelinintoceramideandphosphorylcholine;andisinvolvedinprogrammedcelldeath(apoptosis),celldifferentiationandcellproliferation.Sphingomyelinasesareclassifiedintofivecategories:acidsphingomyelinase(aSMase),secretorysphingomyelinase(sSMase),neutralMg2+/-dependentsphingomyelinases(nSMase),neutralMg2+/-independentsphingomyelinasesandalkalinesphingomyelinase.Acidsphingomyelinasewasthefirstdescribedandbestcharacterizedofthesphingomyelinases.Adeficiencyoflysosomalacidsphingomyelinaseleadstorapidneurodegenerationanddeathduetoexcessiveaccumulationofsphingomyelin(Niemann-Pickdisease).
TheEchelonAcidSphingomyelinaseAssayKitusesafluorogenicsubstratespecificforacidsphingomyelinasetoprovideasensitiveandhomogenousmethodtomeasuretheactivityofaSMaseinvitro.Thekitprovidesallnecessaryreagentstomeasuretheacidsphingomyelinaseactivityof40samplesraninduplicate.

FeaturedinPublication:
1.Li,J.,W.Yu,etal.(2010)."alpha-TEA-induceddeathreceptordependentapoptosisinvolvesactivationofacidsphingomyelinaseandelevatedceramide-enrichedcellsurfacemembranes."CancerCellInt10:40

RelatedProducts:
SphingomyelinaseActivityAssayKit(K-1800)
NeutralSphingomyelinaseAssayService(T-1800)
BiotinSphingomyelin(S-400B)