CatalogNo.: K-3800 Description: ThePI(3,4)P2MassELISAmeasurestheamountofPI(3,4)P2extractedfromcellsbymeansofacompetitiveELISA,(96wellformat). AssayRange:300to1.23pmolPI(3,4)P2 SampleType:Drieddownlipidextractedfromcells SampleVolume:120μL/sample,runinduplicate. | |||
TheassayisacompetitiveELISAwherethesignalisinverselyproportionaltotheamountofPI(3,4)P2produced.ThisallowstheusertomeasuretheamountofPI(3,4)P2extractedfromcellsbymeansofstandard96wellELISAformat,eliminatingtheneedforrADIoactivityandthinlayerchromatography.OncePI(3,4)P2hasbeenextractedfromcellularsamples,itisincubatedwithaPI(3,4)P2detector,thenaddedtothePI(3,4)P2-coatedplateforcompetitivebinding.Aperoxidase-linkedsecondarydetectionreagentandcolorimetricsubstrateisusedtodetectPI(3,4)P2detectorbindingtotheplate.ThecolorimetricsignalisinverselyproportionaltotheamountofPI(3,4)P2extractedfromcells. Format:96-wellCompetitiveELISAStorage:2-8°CRelatedProducts:PIPMassAssaysReferences:1)NonradioactiveQuantificationofPhosphoinositide3-kinase(PI3K)ProductsfromBreastCancerCellLines2.QuantificationofPI3-KinaseProductsfromBreastCancerCellLines Keywords:PI(3,4)P2,PI34P2,PtdIns(3,4)P2 |
Echelon-脂类研究的最佳拍档
Echelon是脂质研究和脂质与蛋白质相互作用研究领域的引导者。脂质蛋白相互作用是众多的细胞信号通路钟高级调控必不可少的。异常的细胞信号通路将会导致许多疾病,例如癌症、炎症、糖尿病和心血管疾病等。Echelon一直是脂质研究信息,脂质信号分析的顶级供应商。