CatalogNo.: T-4200 Description: TheEchelonAutotaxinInhibitorAssayServiceusesoneofEchelon'sAutotaxinInhibitorScreeningkitsdependingonthescaleoftheproject(catalog#'sK-4200orK-4200HTS).ThiskitusesthefluorogenicATXsubstrate,FS-3.FS-3isanLPCanaloguethatisconjugatedwithbothafluorophore(fluorescein)andaquencher.Initsnativestatethequencherinterfereswithfluorescein’sfluorescence.OnceATXcleavesFS-3,fluoresceinbecomesliberatedfromthequencher,resultinginincreasedfluorescence. | |||
Inthisassayservice,twoscreensarerunforeachsample.Intheinitialscreen,recombinantAutotaxinisincubatedwithyoursampleinhibitor(s)andaninternalcontrolinhibitor(BrP-LPA).Thesubstrate,FS-3,isthenaddedsubsequentlyandfluorescenceismeasuredovertime.Theseassaysarecontinuous(notendpoint)andhavetheadvantageofbeinglesssusceptIBLetoerrorarisingfrommistimingintheadditionofreagents.Thesecondaryscreenisperformedtoevaluatewhetherasamplehit,revealedintheinitialscreen,isafalsepositiveduetointerferencewiththeassay.ThesampleinhibitorsthatarepositiveintheinitialscreenandnegativeinthesecondaryscreenarepotentialAutotaxininhibitors. SampleConsiderations:SampleSolvent -Theassayissensitivetoorganicsolvents.Limitto<2%. -TheassayisresistanttomodestconcentrationsofDMSO.Seefigure2onK-4200TDS. -Wewillruncontrolsforallsolventsusedinyoursamplepanel.Thismayaddadditionalcosts. -Itmaybepreferredtosendusvialsofyoursamplesolvent. SampleAmount:60uL/sampleminimumisrequired. Samplesneedtobeinsolutionattheconcentrationstobetested. 品牌介绍
Echelon-脂类研究的最佳拍档 Echelon是脂质研究和脂质与蛋白质相互作用研究领域的引导者。脂质蛋白相互作用是众多的细胞信号通路钟高级调控必不可少的。异常的细胞信号通路将会导致许多疾病，例如癌症、炎症、糖尿病和心血管疾病等。Echelon一直是脂质研究信息，脂质信号分析的顶级供应商。 公司介绍
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